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WHDL - 00015409
A population genetic study of brittle stars was conducted in the Gulf of Mexico to study patterns of gene flow and population structure. Mitochondrial DNA from 85 samples was extracted, amplified, and sequenced using 16S and COI primers. Phylogenetic analyses confirmed the identity of three species, Asteroschema clavigerum and two species exhibiting cryptic speciation: Ophiocreas spinulosus and an unidentified Asteroschema sp. 2. Nested clade phylogeographic analysis and analysis of molecular variance showed that only O. spinulosus had significant genetic structure based on geography or depth and potential isolation by distance. O. spinulosus and the unidentified Asteroschema sp. 2 both showed high levels of gene flow and no population structure. Research of population connectivity in marine species with anthropogenic disturbances is important in understanding species conservation and the effects of habitat change and pollution.
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